mouse naive cd4 t cells Search Results


magcellect mouse naive cd4 t cell isolation kit  (R&D Systems)
92
R&D Systems magcellect mouse naive cd4 t cell isolation kit
Magcellect Mouse Naive Cd4 T Cell Isolation Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/magcellect mouse naive cd4 t cell isolation kit/product/R&D Systems
Average 92 stars, based on 1 article reviews
magcellect mouse naive cd4 t cell isolation kit - by Bioz Stars, 2026-02
92/100 stars
  Buy from Supplier
naive cd4 t cell isolation kit  (Miltenyi Biotec)
99
Miltenyi Biotec naive cd4 t cell isolation kit
A Immunoblot analysis of Ripk1 in purified Treg cells from Ripk1 fl/fl Cre-ER T2 Foxp3 hCD2 and Ripk1 wt/wt Cre-ER T2 Foxp3 hCD2 (control) mice treated with tamoxifen or vehicle for the indicated time points. B Representative dot plots, C Foxp3 + hCD2 + percentages, and D Foxp3 geometric mean fluorescence intensity (gMFI) of events within live <t>CD4</t> + cells of MACS-sorted naïve CD4 + T cells from Ripk1 fl/fl Cre-ER T2 Foxp3 hCD2 ( Ripk1 fl/fl ; n = 4) and Ripk1 wt/wt Cre-ER T2 Foxp3 hCD2 ( Ripk1 wt/wt control; n = 4) mice treated with tamoxifen for 72 h and subsequently with Treg cell polarizing conditions. Data was obtained from four independent experiments. E Representative dot plots (left) and bar graphs (right) of viability analysis determined by CellEvent Caspase-3/7 and LIVE/DEAD Fixable Blue staining of MACS-sorted CD4 + hCD2 + cells from Ripk1 fl/fl Cre-ER T2 Foxp3 hCD2 ( Ripk1 fl/fl ; n = 5) and Ripk1 wt/wt Cre-ER T2 Foxp3 hCD2 ( Ripk1 wt/wt control; n = 5) mice and treated in vitro with Tamoxifen for 72 h. Data was obtained from five independent experiments. F Frequencies of viable cells determined by LIVE/DEAD Fixable Blue staining of MACS-sorted CD4 + hCD2 + cells from Ripk1 fl/fl Cre-ER T2 Foxp3 hCD2 ( Ripk1 fl/fl ; n = 8) and Ripk1 wt/wt Cre-ER T2 Foxp3 hCD2 ( Ripk1 wt/wt control; n = 8) mice and treated in vitro with Tamoxifen for 72 h and, subsequently with TNF (50 ng/ml), anti-CD3 and anti-CD28, combination of anti-CD3, anti-CD28 and TNF (50 ng/ml) or left untreated for 16 additional hours. C – F Data in bar graphs are represented as mean ± SEM. Statistical analyses were performed by two-tailed Mann–Whitney tests. * p < 0.05, n.s. not significant.
Naive Cd4 T Cell Isolation Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/naive cd4 t cell isolation kit/product/Miltenyi Biotec
Average 99 stars, based on 1 article reviews
naive cd4 t cell isolation kit - by Bioz Stars, 2026-02
99/100 stars
  Buy from Supplier
mouse naive cd4 t cell isolation kit  (R&D Systems)
92
R&D Systems mouse naive cd4 t cell isolation kit
A Immunoblot analysis of Ripk1 in purified Treg cells from Ripk1 fl/fl Cre-ER T2 Foxp3 hCD2 and Ripk1 wt/wt Cre-ER T2 Foxp3 hCD2 (control) mice treated with tamoxifen or vehicle for the indicated time points. B Representative dot plots, C Foxp3 + hCD2 + percentages, and D Foxp3 geometric mean fluorescence intensity (gMFI) of events within live <t>CD4</t> + cells of MACS-sorted naïve CD4 + T cells from Ripk1 fl/fl Cre-ER T2 Foxp3 hCD2 ( Ripk1 fl/fl ; n = 4) and Ripk1 wt/wt Cre-ER T2 Foxp3 hCD2 ( Ripk1 wt/wt control; n = 4) mice treated with tamoxifen for 72 h and subsequently with Treg cell polarizing conditions. Data was obtained from four independent experiments. E Representative dot plots (left) and bar graphs (right) of viability analysis determined by CellEvent Caspase-3/7 and LIVE/DEAD Fixable Blue staining of MACS-sorted CD4 + hCD2 + cells from Ripk1 fl/fl Cre-ER T2 Foxp3 hCD2 ( Ripk1 fl/fl ; n = 5) and Ripk1 wt/wt Cre-ER T2 Foxp3 hCD2 ( Ripk1 wt/wt control; n = 5) mice and treated in vitro with Tamoxifen for 72 h. Data was obtained from five independent experiments. F Frequencies of viable cells determined by LIVE/DEAD Fixable Blue staining of MACS-sorted CD4 + hCD2 + cells from Ripk1 fl/fl Cre-ER T2 Foxp3 hCD2 ( Ripk1 fl/fl ; n = 8) and Ripk1 wt/wt Cre-ER T2 Foxp3 hCD2 ( Ripk1 wt/wt control; n = 8) mice and treated in vitro with Tamoxifen for 72 h and, subsequently with TNF (50 ng/ml), anti-CD3 and anti-CD28, combination of anti-CD3, anti-CD28 and TNF (50 ng/ml) or left untreated for 16 additional hours. C – F Data in bar graphs are represented as mean ± SEM. Statistical analyses were performed by two-tailed Mann–Whitney tests. * p < 0.05, n.s. not significant.
Mouse Naive Cd4 T Cell Isolation Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse naive cd4 t cell isolation kit/product/R&D Systems
Average 92 stars, based on 1 article reviews
mouse naive cd4 t cell isolation kit - by Bioz Stars, 2026-02
92/100 stars
  Buy from Supplier
magnetic nanoparticle based isolation kit for mouse naive cd4 + t cells  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc magnetic nanoparticle based isolation kit for mouse naive cd4 + t cells
A Immunoblot analysis of Ripk1 in purified Treg cells from Ripk1 fl/fl Cre-ER T2 Foxp3 hCD2 and Ripk1 wt/wt Cre-ER T2 Foxp3 hCD2 (control) mice treated with tamoxifen or vehicle for the indicated time points. B Representative dot plots, C Foxp3 + hCD2 + percentages, and D Foxp3 geometric mean fluorescence intensity (gMFI) of events within live <t>CD4</t> + cells of MACS-sorted naïve CD4 + T cells from Ripk1 fl/fl Cre-ER T2 Foxp3 hCD2 ( Ripk1 fl/fl ; n = 4) and Ripk1 wt/wt Cre-ER T2 Foxp3 hCD2 ( Ripk1 wt/wt control; n = 4) mice treated with tamoxifen for 72 h and subsequently with Treg cell polarizing conditions. Data was obtained from four independent experiments. E Representative dot plots (left) and bar graphs (right) of viability analysis determined by CellEvent Caspase-3/7 and LIVE/DEAD Fixable Blue staining of MACS-sorted CD4 + hCD2 + cells from Ripk1 fl/fl Cre-ER T2 Foxp3 hCD2 ( Ripk1 fl/fl ; n = 5) and Ripk1 wt/wt Cre-ER T2 Foxp3 hCD2 ( Ripk1 wt/wt control; n = 5) mice and treated in vitro with Tamoxifen for 72 h. Data was obtained from five independent experiments. F Frequencies of viable cells determined by LIVE/DEAD Fixable Blue staining of MACS-sorted CD4 + hCD2 + cells from Ripk1 fl/fl Cre-ER T2 Foxp3 hCD2 ( Ripk1 fl/fl ; n = 8) and Ripk1 wt/wt Cre-ER T2 Foxp3 hCD2 ( Ripk1 wt/wt control; n = 8) mice and treated in vitro with Tamoxifen for 72 h and, subsequently with TNF (50 ng/ml), anti-CD3 and anti-CD28, combination of anti-CD3, anti-CD28 and TNF (50 ng/ml) or left untreated for 16 additional hours. C – F Data in bar graphs are represented as mean ± SEM. Statistical analyses were performed by two-tailed Mann–Whitney tests. * p < 0.05, n.s. not significant.
Magnetic Nanoparticle Based Isolation Kit For Mouse Naive Cd4 + T Cells, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/magnetic nanoparticle based isolation kit for mouse naive cd4 + t cells/product/STEMCELL Technologies Inc
Average 90 stars, based on 1 article reviews
magnetic nanoparticle based isolation kit for mouse naive cd4 + t cells - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier

Image Search Results


A Immunoblot analysis of Ripk1 in purified Treg cells from Ripk1 fl/fl Cre-ER T2 Foxp3 hCD2 and Ripk1 wt/wt Cre-ER T2 Foxp3 hCD2 (control) mice treated with tamoxifen or vehicle for the indicated time points. B Representative dot plots, C Foxp3 + hCD2 + percentages, and D Foxp3 geometric mean fluorescence intensity (gMFI) of events within live CD4 + cells of MACS-sorted naïve CD4 + T cells from Ripk1 fl/fl Cre-ER T2 Foxp3 hCD2 ( Ripk1 fl/fl ; n = 4) and Ripk1 wt/wt Cre-ER T2 Foxp3 hCD2 ( Ripk1 wt/wt control; n = 4) mice treated with tamoxifen for 72 h and subsequently with Treg cell polarizing conditions. Data was obtained from four independent experiments. E Representative dot plots (left) and bar graphs (right) of viability analysis determined by CellEvent Caspase-3/7 and LIVE/DEAD Fixable Blue staining of MACS-sorted CD4 + hCD2 + cells from Ripk1 fl/fl Cre-ER T2 Foxp3 hCD2 ( Ripk1 fl/fl ; n = 5) and Ripk1 wt/wt Cre-ER T2 Foxp3 hCD2 ( Ripk1 wt/wt control; n = 5) mice and treated in vitro with Tamoxifen for 72 h. Data was obtained from five independent experiments. F Frequencies of viable cells determined by LIVE/DEAD Fixable Blue staining of MACS-sorted CD4 + hCD2 + cells from Ripk1 fl/fl Cre-ER T2 Foxp3 hCD2 ( Ripk1 fl/fl ; n = 8) and Ripk1 wt/wt Cre-ER T2 Foxp3 hCD2 ( Ripk1 wt/wt control; n = 8) mice and treated in vitro with Tamoxifen for 72 h and, subsequently with TNF (50 ng/ml), anti-CD3 and anti-CD28, combination of anti-CD3, anti-CD28 and TNF (50 ng/ml) or left untreated for 16 additional hours. C – F Data in bar graphs are represented as mean ± SEM. Statistical analyses were performed by two-tailed Mann–Whitney tests. * p < 0.05, n.s. not significant.

Journal: Cell Death and Differentiation

Article Title: Ripk1 is critical for preserving effector regulatory T cells and the suppressive transcriptional program in regulatory T cells

doi: 10.1038/s41418-025-01550-3

Figure Lengend Snippet: A Immunoblot analysis of Ripk1 in purified Treg cells from Ripk1 fl/fl Cre-ER T2 Foxp3 hCD2 and Ripk1 wt/wt Cre-ER T2 Foxp3 hCD2 (control) mice treated with tamoxifen or vehicle for the indicated time points. B Representative dot plots, C Foxp3 + hCD2 + percentages, and D Foxp3 geometric mean fluorescence intensity (gMFI) of events within live CD4 + cells of MACS-sorted naïve CD4 + T cells from Ripk1 fl/fl Cre-ER T2 Foxp3 hCD2 ( Ripk1 fl/fl ; n = 4) and Ripk1 wt/wt Cre-ER T2 Foxp3 hCD2 ( Ripk1 wt/wt control; n = 4) mice treated with tamoxifen for 72 h and subsequently with Treg cell polarizing conditions. Data was obtained from four independent experiments. E Representative dot plots (left) and bar graphs (right) of viability analysis determined by CellEvent Caspase-3/7 and LIVE/DEAD Fixable Blue staining of MACS-sorted CD4 + hCD2 + cells from Ripk1 fl/fl Cre-ER T2 Foxp3 hCD2 ( Ripk1 fl/fl ; n = 5) and Ripk1 wt/wt Cre-ER T2 Foxp3 hCD2 ( Ripk1 wt/wt control; n = 5) mice and treated in vitro with Tamoxifen for 72 h. Data was obtained from five independent experiments. F Frequencies of viable cells determined by LIVE/DEAD Fixable Blue staining of MACS-sorted CD4 + hCD2 + cells from Ripk1 fl/fl Cre-ER T2 Foxp3 hCD2 ( Ripk1 fl/fl ; n = 8) and Ripk1 wt/wt Cre-ER T2 Foxp3 hCD2 ( Ripk1 wt/wt control; n = 8) mice and treated in vitro with Tamoxifen for 72 h and, subsequently with TNF (50 ng/ml), anti-CD3 and anti-CD28, combination of anti-CD3, anti-CD28 and TNF (50 ng/ml) or left untreated for 16 additional hours. C – F Data in bar graphs are represented as mean ± SEM. Statistical analyses were performed by two-tailed Mann–Whitney tests. * p < 0.05, n.s. not significant.

Article Snippet: Naïve CD4 + T cells were isolated from pooled spleen and lymph node single-cell suspensions from Ripk1 fl/fl Cre-ER T2 Foxp3 hCD2 and Ripk1 wt/wt Cre-ER T2 Foxp3 hCD2 mice using Naive CD4 + T Cell Isolation kit (130-104-453, Miltenyi Biotec) according to manufacturer ́s instructions.

Techniques: Western Blot, Purification, Control, Fluorescence, Staining, In Vitro, Two Tailed Test, MANN-WHITNEY